FastQCFastQC Report
Tue 3 May 2016
SRR3192396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3192396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105089150
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA5497210.5230996729919312No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT5142410.48933786218653397No Hit
TTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCA4936080.46970405603242577No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC4483180.42660731388540113No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT4232160.40272092789788483No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2985540.28409593188259685No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2442730.23244359669861253No Hit
CACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTC2381730.22663900126701947No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG2330200.22173554548685567No Hit
CAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTT1982130.18861414332497695No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGAT1970400.18749794817067225No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1914390.1821681876768439No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1798730.17116229410933478No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA1697180.16149907007526465No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1594120.1516921585149371No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1509320.14362281929200113No Hit
AATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGA1353330.12877923172848957No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG1315070.12513851334795267No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG1295380.12326486606847614No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA1292960.12303458539725558No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA1289350.12269106753646786No Hit
GGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC1211690.11530115145093475No Hit
GCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCC1132960.10780941705209339No Hit
GCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGA1090680.1037861663168843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTCGC271700.026.1998677
CTCGCTA901300.024.1103618
TCGCTAT941000.023.4211949
GATGTGT773900.022.3604852
GTCTCGC1013000.021.7377576
GGGGTCT2234000.021.6014923
TAAGCGT383850.021.23180682-83
CCCTACG676450.020.53985832-33
CTACGTT687050.020.28531334-35
GGATGTG870150.020.0312461
CACTTCG367300.019.7814256
TCTCGCT1125850.019.740247
GGGGGTC1492900.019.2224862
GGTCTTA1232550.019.2103315
GGTCTCG1179100.019.122615
CTTCGCT390150.018.8298038
GGGGGGT820100.018.6849231
TTCGCTG390850.018.6502829
ACTACCC751600.018.54490328-29
AACGAAC882750.018.45718876-77
Produced by FastQC (version 0.11.2)